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KMID : 1007520030120040435
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Food Science and Biotechnology
2003 Volume.12 No. 4 p.435 ~ p.438
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Improvement of Detection Sensitivity of Listeria monocytogenes Using Crude Pediocin PA-1 by Polymerase Chain Reaction (PCR)
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Moon, Gi Seong
Kim, Wang June/Shin, Weon-sun
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Abstract
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Listeria monocytogenes is a gram-positive bacteria with a lysis resistant cell wall composed of rigid peptidoglycan. In general, L. monocytogenes cna be detected at the 10^(３) cell level usng a species-specific primer set from artificially contaminated food samples. When treated with 160 AU of crude pediocin PA-1, which is an antilisterial bacteriocin produced by Pediococcus acidilactici K10, the detection limit of L. monocytogenes was reduced to 10^(１) and 10^(２) cells from artificially contaminated raw pork and whole milk, respectively. By using crude pediocin PA-1, the cell membrane of the pathogen was effectively disrupted to release DNA, indicating an increase in susceptibility to PCR detection. The total assay time for the food-borne L. monocytogenes treated with the crude pediocin PA-1 was ＜4 hr without enrichment at a high sensitivity.
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